Based on the new gas-shearing strategy, a simple one-step and oil-free process, based on the gas-flow-assisted formation of microdroplets (‘‘gas-shearing’’), is established for the scalable production of monodisperse multi-compartmental microspheres (MCMs). As oils and surfactants are not used, the gas-shearing method is highly cytocompatible. The versatile applications of such MCMs are demonstrated by producing a microrobot, a biocompatible carrier for the coculturing of cells, drug anti-counterfeiting, etc. Which may become attractive materials for biomedical applications.